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Resources: Infrastructure

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Call for new TCNP Driving Biology Projects (DBPs)

The CMU-Pitt National Technology Center for Networks & Pathways (TCNP) on Fluorescent Probes and Imaging plans to add three to five new Driving Biological Projects (DBPs) to its program in the final two years. The focus of our center is on the development of novel fluorescent probes to interrogate signaling pathways in living cells, in real time. This is an NIH Roadmap funded center based at Carnegie Mellon University and the University of Pittsburgh. Core 2 of this TCNP currently consists of four 3-year DBPs which will be transitioning to R01 or other support in the next few months. Two years of funding for new DBPs will become available on or about August 1, 2008 and we welcome applications for this funding. This funding will be committed for two years, but continued funding into the second year is dependent on evaluation of progress at the end of year 1.

For information about the NIH Roadmap National Technology Centers for Networks and Pathways, please see: http://nihroadmap.nih.gov/buildingblocks/technologycenters/

For detailed information, please download the RFA in either Word Document or PDF format.

TCNP Imaging Resource: Center for Biologic Imaging

Through the the National Technology Center for Networks and Pathways collaborative program MBIC users have access to the imaging resources at the Center for Biologic Imaging at the University of Pittsburgh. The following link will take you to the CBI booking system for their fluorescence, confocal and electron microscopy resources.

Center for Biologic Imaging Scheduling System

MBIC Infrastructure: On-line Booking System

We are now starting to operate an on-line booking system for shared equipment and resources within MBIC. Each of the links below will take you to the calendar for a given instrument. Once at the calendar you will be able to see bookings for each day. In order to make a booking, click on the date link at the top of each day and that will allow an individual booking to be made.

Before you can view the calendar and make a booking you must authenticate, and in order to do that you must become a registered user by filling out the user request form. User accounts are usually created within a couple of hours, but requests may occasionally take upto 24 hours to be processed.

BSL-1 Cell culture hoods

  1. Culture Hood 1 (by door of cell culture room)
  2. Culture Hood 2 (around the corner of cell culture room)

Fluorescence readers & microscopes

  1. Tecan Safire2 Fluorescence Plate Reader
  2. Carl Zeiss Axio Imaging 2 ApoTome Upright Fluorescence Microscope
  3. Carl Zeiss Axiovert 135 Inverted Single Molecule Microscope (Restricted - Bruchez Group)
  4. Beckman Coulter IC-100 Image Cytometer (Restricted - Murphy Group)
  5. Cellomics Kinetics Scan Reader HTS system (Restricted - Murphy Group)

Confocal Microscopes

  1. Carl Zeiss LSM 510 Meta NLO Confocor 3 Inverted Spectral Confocal Microscope
  2. Carl Zeiss LSM 510 Meta/ UV DuoScan Inverted Spectral Confocal Microscope
  3. Olympus Fluoview 300 Multiphoton Inverted Confocal Microscope
  4. Olympus Spinning Disc Inverted Confocal Microscope (Restricted - Jarvik Group)